Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [2]
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Validation data
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- Product number
- PA5-15004 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ALDH3A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Controlling the 3D architecture of Self-Lifting Auto-generated Tissue Equivalents (SLATEs) for optimized corneal graft composition and stability.
Template Curvature Influences Cell Alignment to Create Improved Human Corneal Tissue Equivalents.
Gouveia RM, González-Andrades E, Cardona JC, González-Gallardo C, Ionescu AM, Garzon I, Alaminos M, González-Andrades M, Connon CJ
Biomaterials 2017 Mar;121:205-219
Biomaterials 2017 Mar;121:205-219
Template Curvature Influences Cell Alignment to Create Improved Human Corneal Tissue Equivalents.
Gouveia RM, Koudouna E, Jester J, Figueiredo F, Connon CJ
Advanced biosystems 2017 Dec;1(12):e1700135
Advanced biosystems 2017 Dec;1(12):e1700135
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using an ALDH3A1 polyclonal antibody (Product # PA5-15004) in Y79 cell lysates (35 µg per lane).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using an ALDH3A1 polyclonal antibody (Product # PA5-15004) in mouse muscle tissue lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue using an ALDH3A1 polyclonal antibody (Product # PA5-15004), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Protein composition of curved corneal stromal tissue equivalents. Curved corneal tissues were imaged by immunofluorescence confocal microscopy, with tridimensional reconstruction of 630 x 630 um areas of tissue analyzed for expression of a) collagen I and keratocan, b) collagen V and lumican, c) ALDH1 and decorin, d) ALDH3 and fibronectin, and e) CHST6 and alphaSMA. Cell nuclei were identified by DAPI staining. Signal specificity was evaluated using f) secondary antibody-only controls.