TA322903

antibody from OriGene

Targeting: SPAG4 CT127, SUN4

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA322903

Provider

OriGene

Product name

Anti-SPAG4 Rabbit Polyclonal Antibody

Antibody type

Polyclonal

Description

Anti-SPAG4 Rabbit Polyclonal Antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

SPAG4

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µl

Concentration

1.6 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted band size: 48 kDa . Positive control: Hela and K562 cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: Hela cell lysate Lane1: K562 cell lysate Lysate: 40 ug per lane Primary antibody: 1/1100 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted cell location: Cytoplasm. Positive control: Human ovarian cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using SPAG4 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)

Validation comment

IHC