Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- GTX24476 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX24476, RRID:AB_380381
- Product name
- LAT (phospho Tyr132) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references GRB2 Nucleates T Cell Receptor-Mediated LAT Clusters That Control PLC-γ1 Activation and Cytokine Production.
Novel CD3-specific antibody induces immunosuppression via impaired phosphorylation of LAT and PLCγ1 following T-cell stimulation.
Bilal MY, Houtman JC
Frontiers in immunology 2015;6:141
Frontiers in immunology 2015;6:141
Novel CD3-specific antibody induces immunosuppression via impaired phosphorylation of LAT and PLCγ1 following T-cell stimulation.
Shiheido H, Aoyama T, Takahashi H, Hanaoka K, Abe T, Nishida E, Chen C, Koga O, Hikida M, Shibagaki Y, Morita A, Nikawa T, Hattori S, Watanabe T, Shimizu J
European journal of immunology 2014 Jun;44(6):1770-80
European journal of immunology 2014 Jun;44(6):1770-80
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Extracts prepared from Jurkat E6.1 cells were left unstimulated (1) or stimulated (2-5), resolved by SDS-PAGE on a 10% polyacrylamide gel, and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4¢XC.