100-401-962

antibody from Rockland Immunochemicals, Inc.

Targeting: EIF2AK3 PEK, PERK

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

100-401-962

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#100-401-962, RRID:AB_2095866

Product name

Anti-PKR-like Endoplasmic Reticulum Kinase (PERK) (RABBIT) Antibody - 100-401-962

Antibody type

Polyclonal

Vial size

100 µl

References

Submitted references

Exposure to binge ethanol and fatty acid ethyl esters exacerbates chronic ethanol-induced pancreatic injury in hepatic alcohol dehydrogenase-deficient deer mice.

Srinivasan MP, Bhopale KK, Caracheo AA, Kaphalia L, Gong B, Popov VL, Boor PJ, Shakeel Ansari GA, Kaphalia BS
American journal of physiology. Gastrointestinal and liver physiology 2022 Mar 1;322(3):G327-G345

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Activation of AMP-activated protein kinase attenuates ethanol-induced ER/oxidative stress and lipid phenotype in human pancreatic acinar cells.

Srinivasan MP, Bhopale KK, Caracheo AA, Amer SM, Khan S, Kaphalia L, Loganathan G, Balamurugan AN, Kaphalia BS
Biochemical pharmacology 2020 Oct;180:114174

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Linking Dysregulated AMPK Signaling and ER Stress in Ethanol-Induced Liver Injury in Hepatic Alcohol Dehydrogenase Deficient Deer Mice.

Srinivasan MP, Bhopale KK, Amer SM, Wan J, Kaphalia L, Ansari GS, Kaphalia BS
Biomolecules 2019 Oct 2;9(10)

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Ethanol Exposure Impairs AMPK Signaling and Phagocytosis in Human Alveolar Macrophages: Role of Ethanol Metabolism.

Kaphalia L, Srinivasan MP, Kakumanu RD, Kaphalia BS, Calhoun WJ
Alcoholism, clinical and experimental research 2019 Aug;43(8):1682-1694

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ATF7 mediates TNF-α-induced telomere shortening.

Maekawa T, Liu B, Nakai D, Yoshida K, Nakamura KI, Yasukawa M, Koike M, Takubo K, Chatton B, Ishikawa F, Masutomi K, Ishii S
Nucleic acids research 2018 May 18;46(9):4487-4504

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A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer.

Howley BV, Link LA, Grelet S, El-Sabban M, Howe PH
Oncogene 2018 Mar;37(10):1308-1325

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miR-216b regulation of c-Jun mediates GADD153/CHOP-dependent apoptosis.

Xu Z, Bu Y, Chitnis N, Koumenis C, Fuchs SY, Diehl JA
Nature communications 2016 May 13;7:11422

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Casitas B-cell lymphoma (Cbl) proteins protect mammary epithelial cells from proteotoxicity of active c-Src accumulation.

Mukhopadhyay C, Triplett A, Bargar T, Heckman C, Wagner KU, Naramura M
Proceedings of the National Academy of Sciences of the United States of America 2016 Dec 20;113(51):E8228-E8237

Immunohistochemistry

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Immunohistochemistry staining of mouse mammary gland samples from lactating mice (L10) with Rockland Immunochemical’s anti-PERK. Positive staining signal observed in wild type mouse sample with anti-PERK staining only (middle image), but not in the knock out mouse sample (right image) and pre-immune serum staining (left image) The anti-PERK was diluted 1:1,000 in 5% goat serum in PBS and allowed to incubate for 2h at room temperature in a humidified chamber. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.

Validation comment

Immunohistochemistry