Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 649401 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#649401, RRID:AB_10642966
- Product name
- Purified anti-PERK Phospho (Ser713)
- Antibody type
- Polyclonal
- Antigen
- Modified synthetic peptide
- Description
- PERK (protein kinase-like endoplasmic reticulum kinase) is a type I membrane protein located in the endoplasmic reticulum (ER). ER stress increases the activity of PERK, which then phosphorylates eIF2α to promote reduced translation, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. PERK-deficient mice have defects in pancreatic β cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress. PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain. Phosphorylation of PERK at Ser713 can be used as a marker for its activation status.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µl
- Storage
- Upon receipt, store at -20°C.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- 293E cells were transfected with PERK-FLAG construct. Cell lysates were then immunoprecipitated with L5 antibody. Proteins were then treated with calf intestinal alkaline phosphatase (CIP). The proteins without CIP treatment were used as the control. Samples were separated by electrophoresis, transferred to nitrocellulose and probed with anti-PERK phospho (Ser713) antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminenescent system.
- Conjugate
- Unconjugated