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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB10175 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10175, RRID:AB_1674971
- Product name
- Eif2ak3 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against partial recombinant Eif2ak3.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Nrf2 as a novel molecular target for chemoprevention.
Nrf2 is a direct PERK substrate and effector of PERK-dependent cell survival.
Dimerization and release of molecular chaperone inhibition facilitate activation of eukaryotic initiation factor-2 kinase in response to endoplasmic reticulum stress.
Lee JS, Surh YJ
Cancer letters 2005 Jun 28;224(2):171-84
Cancer letters 2005 Jun 28;224(2):171-84
Nrf2 is a direct PERK substrate and effector of PERK-dependent cell survival.
Cullinan SB, Zhang D, Hannink M, Arvisais E, Kaufman RJ, Diehl JA
Molecular and cellular biology 2003 Oct;23(20):7198-209
Molecular and cellular biology 2003 Oct;23(20):7198-209
Dimerization and release of molecular chaperone inhibition facilitate activation of eukaryotic initiation factor-2 kinase in response to endoplasmic reticulum stress.
Ma K, Vattem KM, Wek RC
The Journal of biological chemistry 2002 May 24;277(21):18728-35
The Journal of biological chemistry 2002 May 24;277(21):18728-35
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using Eif2ak3 polyclonal antibody (Cat # PAB10175) to detect Eif2ak3 in cell lysates. 300 ug Eif2ak3 over-expressing 293T cell lysate (lanes 1 & 2), or 800 ug wild type (Lanes 3 & 4), and Eif2ak3 knock out (lanes 5 & 6) MEF cell lysate were immunoprecipated with 15 uL anti-Eif2ak3, followed by immunobloting with 1:1000 dilution of Eif2ak3 polyclonal antibody in 5% milk/TBST buffer.Lane 1, 293T cells over-expressing Myc-Eif2ak3 wt, Lane 2, 293T cells over-expressing Myc-Eif2ak3 K618A.Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry staining of mouse mammary gland samples from lactating mice (L10) with Eif2ak3 polyclonal antibody (Cat # PAB10175).Positive staining signal observed in wild type mouse sample with Eif2ak3 polyclonal antibody staining only (B) , but not in the knock out mouse sample (C) and pre-immune serum staining (A) .The Eif2ak3 polyclonal antibody (Cat # PAB10175) was diluted in 1 : 1,000.Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
