AP09082SU-N

antibody from Acris Antibodies GmbH

Targeting: EIF2AK3 PEK, PERK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP09082SU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09082SU-N, RRID:AB_2035838

Product name

anti EIF2AK3 (601-1115)

Antibody type

Polyclonal

Antigen

Recombinant fusion protein from amino acids 601-1115 of Mouse deltaN PERK

Reactivity

Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

85 mg/ml (by Refractometry)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis: AP09082SU-N PERK antibody staining of cell lysates. 300µg PERK over-expressing 293T cell lysate (Lanes 1 and 2), or 800µg wild type (Lanes 3 and 4), and PERK knock out (Lanes 5 and 6) MEF cell lysate were immunoprecipated with 15µl anti-PERK, followed by immunobloting with 1/1000 dilution of anti-PERK in 5% milk/TBST buffer. Lane 1, 293T cells over-expressing Myc-PERK wt, Lane 2 , 293T cells over-expressing Myc-PERK K618A. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry staining of Mouse mammary gland samples from lactating mice (L10) using AP09082SU-N PERK antibody. Positive staining signal observed in wild type Mouse sample with anti-PERK staining only (middle image), but not in the knock out mouse sample (Right image) and pre-immune serum staining (Left image). The anti-PERK was diluted 1/1,000 in 5% goat serum in PBS and allowed to incubate for 2h at RT in a humidified chamber. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.