17-9881-42

antibody from Invitrogen Antibodies

Targeting: HLA-DQB1 CELIAC1, HLA-DQB, IDDM1

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

17-9881-42

Provider

Invitrogen Antibodies

Product name

HLA-DQ Monoclonal Antibody (SK10), APC, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: This SK10 monoclonal antibody reacts with the human MHC Class II HLA-DQ molecule. This cell surface receptor is a heterodimeric protein composed of an alpha (HLA-DQA1) and beta (HLA-DQB1) subunit. HLA-DQ is expressed on antigen presenting cells such as B cells, monocytes, macrophages, dendritic cells, and activated T cells. HLA-DQ plays a role in presenting peptide antigens to CD4+ T lymphocytes. Reports indicate that certain alleles of HLA-DQ are associated with celiac disease and type I diabetes.

Antibody clone number

SK10

Concentration

5 µL/Test

References

Submitted references

Celiac Disease Defined by Over-Sensitivity to Gliadin Activation and Superior Antigen Presentation of Dendritic Cells.

Hudec M, Riegerová K, Pala J, Kútna V, Černá M, O Leary VB
International journal of molecular sciences 2021 Sep 15;22(18)

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Characteristic patterns of HLA presentation and T cell differentiation in adult-onset Still's disease.

Jung JY, Choi B, Sayeed HM, Suh CH, Kim YW, Kim HA, Sohn S
International journal of immunopathology and pharmacology 2018 Jan-Dec;32:2058738418791284

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A genetic perspective on coeliac disease.

Trynka G, Wijmenga C, van Heel DA
Trends in molecular medicine 2010 Nov;16(11):537-50

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A new automated human leukocyte antigen genotyping strategy to identify DR-DQ risk alleles for celiac disease and type 1 diabetes mellitus.

Lavant EH, Carlson JA
Clinical chemistry and laboratory medicine 2009;47(12):1489-95

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Activation pathways implicate anti-HLA-DP and anti-LFA-1 antibodies as lead candidates for intervention in chronic berylliosis.

Chou YK, Edwards DM, Weinberg AD, Vandenbark AA, Kotzin BL, Fontenot AP, Burrows GG
Journal of immunology (Baltimore, Md. : 1950) 2005 Apr 1;174(7):4316-24

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Increased endothelial expression of HLA-DQ and interleukin 1alpha in extra-articular rheumatoid arthritis. Results from immunohistochemical studies of skeletal muscle.

Turesson C, Englund P, Jacobsson LT, Sturfelt G, Truedsson L, Nennesmo I, Lundberg IE
Rheumatology (Oxford, England) 2001 Dec;40(12):1346-54

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human HLA-DQ APC (purple histogram). Cells in the lymphocyte gate were used for analysis.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Supplement Figure 1. Representative examples of flow cytometric histograms of surface-stained cells presenting human leukocyte antigen (HLA)-DQ from the peripheral blood of one patient with adult-onset Still's disease (AOSD), a patient with rheumatoid arthritis (RA), and a healthy control (HC).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1. Flow cytometric results: the percentage of surface-stained cells presenting HLA-DP, DR, and DQ in AOSD patients, RA patients, and HCs. Results were obtained from 14 patients with adult-onset Still's disease (AOSD), 20 with rheumatoid arthritis (RA), and 20 healthy controls (HC). The horizontal line indicates the mean value for each group. P values were determined by the Mann-Whitney U test.