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antibody from Invitrogen Antibodies
Targeting: TLR5
FLJ10052, MGC126430, MGC126431, SLEB1, TIL3
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [2]
- Other assay [2]
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- Product number
- PA1-41139 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TLR5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references TLR5 participates in the TLR4 receptor complex and promotes MyD88-dependent signaling in environmental lung injury.
Flagellin/TLR5 signalling activates renal collecting duct cells and facilitates invasion and cellular translocation of uropathogenic Escherichia coli.
Hussain S, Johnson CG, Sciurba J, Meng X, Stober VP, Liu C, Cyphert-Daly JM, Bulek K, Qian W, Solis A, Sakamachi Y, Trempus CS, Aloor JJ, Gowdy KM, Foster WM, Hollingsworth JW, Tighe RM, Li X, Fessler MB, Garantziotis S
eLife 2020 Jan 28;9
eLife 2020 Jan 28;9
Flagellin/TLR5 signalling activates renal collecting duct cells and facilitates invasion and cellular translocation of uropathogenic Escherichia coli.
Bens M, Vimont S, Ben Mkaddem S, Chassin C, Goujon JM, Balloy V, Chignard M, Werts C, Vandewalle A
Cellular microbiology 2014 Oct;16(10):1503-17
Cellular microbiology 2014 Oct;16(10):1503-17
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TLR5 in (A) Ramos, (B) RAW, (C) mouse spleen, (D) mouse lung and (E) rat lung lysate using a TLR5 polyclonal antibody (Product # PA1-41139) at 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-TLR5 Polyclonal Antibody (Product # PA1-41139) and a 93 kDa band corresponding to Toll-like receptor 5 was observed across all the cell lines tested. 30 µg lysates of HT-29 (Lane 1), U-937 (Lane 2), A549 (Lane 3), HeLa (Lane 4), Caco-2 (Lane 5) and HaCaT (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB24001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody at a concentration of 1 µg/mL and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 diultion) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescentdetection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Toll-like receptor 5 was achieved by transfecting HT-29 with Toll-like receptor 5 specific siRNAs (Silencer® select Product # s14199, s14197). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Toll-like receptor 5 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1) along with an uncharacterized band (*). The blot was probed with TLR5 Polyclonal Antibody (Product # PA1-41139, at a concentration of 1 µg/mL ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in the histogram (Fig. b). A decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Toll-like receptor 5.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TLR5 in formalin-fixed paraffin-embedded human stomach tumor tissue. Samples were incubated in TLR5 polyclonal antibody (Product # PA1-41139) using a dilution of 10 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Intracellular flow analysis of TLR5 in Balb/c mouse splenocytes using a TLR5 polyclonal antibody (Product # PA1-41139) at 2 µg/10^6 cells. Shaded histogram represents cells without antibody; green represents rabbit IgG isotype control; red represents TLR5 polyclonal antibody (Product # PA1-41139). Goat anti-rabbit PE was used as secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of TLR5 in Balb/c mouse splenocytes. Samples were incubated in TLR5 polyclonal antibody (Product # PA1-41139) using a dilution of 2 µg/10^6 cells followed by Goat anti-rabbit PE secondary antibody. Shaded histogram: cells without antibody. Green: rabbit IgG isotype control. Red: TLR5 antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 8. TLR5 participates in TLR4 signaling complex. ( A ) Co-immunoprecipitation of hemagglutinin-tagged TLR5 (TLR5-HA) and FLAG-tagged TLR4 (TLR4-FLAG) in HEK293 cells after 100 ng/mL ultrapure LPS exposure. ( B ) Immunoprecipitation of TLR5 with biotinylated ultrapure LPS (Biotin-LPS) in TLR5-HA and TLR4-FLAG transfected HEK293 cells after 100 ng/mL Biotin-LPS exposure for 15 min. Representative of 2 separate experiments. Figure 8--figure supplement 1. Cell surface expression of TLR4 and its co-localization with TLR5.
