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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB6704 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TLR5 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human TLR5 in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) TLR1, 2, 3, 4, 7, 8, recombinant mouse TLR5, 6, or rhTIRAP is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
O60602- Isotype
- IgG
- Antibody clone number
- 624915
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TLR5 in Human Intestine. TLR5 was detected in immersion fixed paraffin-embedded sections of human intestine using Mouse Anti-Human TLR5 Monoclonal Antibody (Catalog # MAB6704) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panels show a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TLR5 in Human Human Peripheral Blood Monocytes by Flow Cytometry. Human Human Peripheral Blood Monocytes were stained with (A) Mouse Anti-Human TLR5 Monoclonal Antibody (Catalog # MAB6704) or (B) isotype control antibody (Catalog # MAB002), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). View our protocol for Staining Membrane-associated Proteins.
