Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX117706 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX117706, RRID:AB_10729301
- Product name
- ABCE1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Eukaryotic elongation factor 2 kinase regulates the cold stress response by slowing translation elongation.
Knight JR, Bastide A, Roobol A, Roobol J, Jackson TJ, Utami W, Barrett DA, Smales CM, Willis AE
The Biochemical journal 2015 Jan 15;465(2):227-38
The Biochemical journal 2015 Jan 15;465(2):227-38
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ABCE1 antibody detects ABCE1 protein by Western blot analysis.A. 30 ?g K562 whole cell lysate/extract7.5 % SDS-PAGEABCE1 antibody (GTX117706) dilution: 1:500
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ABCE1 antibody (GTX117706) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ABCE1 antibody detects ABCE1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37¢J for 30 min.Green: ABCE1 protein stained by ABCE1 antibody (GTX117706) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ABCE1(GTX117706) antibody at 1:500 dilution.
