TA501500

antibody from OriGene

Targeting: DYNC1LI1 DNCLI1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501500

Provider

OriGene

Proper citation

OriGene Cat#TA501500, RRID:AB_11140305

Product name

Anti-DYNC1LI1 mouse monoclonal antibody, clone OTI1D1 (formerly 1D1)

Antibody type

Monoclonal

Description

Anti-DYNC1LI1 mouse monoclonal antibody, clone OTI1D1 (formerly 1D1)

Host

Mouse

Conjugate

Unconjugated

Epitope

DYNC1LI1

Isotype

IgG

Antibody clone number

OTI1D1

Vial size

100 µl

Concentration

0.51 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DYNC1LI1 (RC222010, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DYNC1LI1.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DYNC1LI1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-DYNC1LI1 mouse monoclonal antibody (TA501500) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DYNC1LI1(RC222010).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC222010 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DYNC1LI1 antibody(TA501500), and then analyzed by flow cytometry.

Validation comment

FC