PA5-44105

antibody from Invitrogen Antibodies

Targeting: ISYNA1 Ino1, INOS, IPS

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-44105

Provider

Invitrogen Antibodies

Product name

ISYNA1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide sequence: LQEQLWPHME ALRPRPSVYI PEFIAANQSA RADNLIPGSR AQQLEQIRRD

Concentration

0.5 mg/mL

References

Submitted references

Phosphatidic acid inhibits inositol synthesis by inducing nuclear translocation of kinase IP6K1 and repression of myo-inositol-3-P synthase.

Lazcano P, Schmidtke MW, Onu CJ, Greenberg ML
The Journal of biological chemistry 2022 Sep;298(9):102363

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The sodium/myo-inositol co-transporter SLC5A3 promotes non-small cell lung cancer cell growth.

Cui Z, Mu C, Wu Z, Pan S, Cheng Z, Zhang ZQ, Zhao J, Xu C
Cell death & disease 2022 Jun 27;13(6):569

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Regulated inositol synthesis is critical for balanced metabolism and development in Drosophila melanogaster.

Rivera MJ, Contreras A, Nguyen LT, Eldon ED, Klig LS
Biology open 2021 Oct 15;10(10)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human adult placenta cells using an anti-ISYNA1 polyclonal antibody (Product # PA5-44105).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human HepG2 cells using an anti-ISYNA1 polyclonal antibody (Product # PA5-44105).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human lung cells using an anti-ISYNA1 polyclonal antibody (Product # PA5-44105).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 3. Inos mRNA levels vary with promoter-GAL4 constructs and MIPS protein levels vary in concordance with Inos mRNA levels. (A) The D. melanogaster Inos gene and the surrounding genomic region of chromosome 2. Location of the P-element D{XP+} is indicated by a green triangle. The primers for exon 1 to exon 2 (qRT-PCR experiments) are blue arrows. The details of the XP+ element are not to scale. Exons are blue blocks, introns are black lines, UTRs are grey blocks. (B) qRT-PCR experiments with larvae ( N >=10 per experimental condition per trial) grown on standard rich food (inositol supplementation as indicated). Normalized to RPL32. Three independent trials of (control) strain ActGal4-3/+ were indistinguishable from the wild-type control Canton-S results shown. Mean+-s.e. of three-six independent trials are represented. * P