H00005688-M01

antibody from Abnova Corporation

Targeting: PSMA7 C6, HSPC, RC6-1, XAPC7

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005688-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005688-M01, RRID:AB_425628

Product name

PSMA7 monoclonal antibody (M01), clone 1A10-3G12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant PSMA7.

Antigen sequence

MSYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGV
RGRDIVVLGVEKKSVAKLQDERTVRKICALDDNVC
MAFAGLTADARIVINRARVECQSHRLTVEDPVTVE
YITRYIASLKQRYTQSNGRRPFGISALIVGFDFDG
TPRLYQTDPSGTYHAWKANAIGRGAKSVREFLEKN
YTDEAIETDDLTIKLVIKALLEVVQSGGKNIELAV
MRRDQSLKILNPEEIEKYVAEIEKEKEENEKKKQK
KAS


Isotype

IgG

Antibody clone number

1A10-3G12

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

PSMA7 inhibits the tumorigenicity of A549 human lung adenocarcinoma cells.

Tan JY, Huang X, Luo YL
Molecular and cellular biochemistry 2012 Jul;366(1-2):131-7

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Proteomic identification of putative biomarkers of radiotherapy resistance: a possible role for the 26S proteasome?

Smith L, Qutob O, Watson MB, Beavis AW, Potts D, Welham KJ, Garimella V, Lind MJ, Drew PJ, Cawkwell L
Neoplasia (New York, N.Y.) 2009 Nov;11(11):1194-207

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

PSMA7 monoclonal antibody (M01), clone 1A10-3G12. Western Blot analysis of PSMA7 expression in human omentum, serous carcinoma.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

PSMA7 monoclonal antibody (M01), clone 1A10-3G12 Western Blot analysis of PSMA7 expression in HeLa ( Cat # L013V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PSMA7 expression in transfected 293T cell line by PSMA7 monoclonal antibody (M01), clone 1A10-3G12.Lane 1: PSMA7 transfected lysate (Predicted MW: 27.9 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PSMA7 is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to PSMA7 on HeLa cell. [antibody concentration 1 ~ 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of PSMA7 transfected lysate using anti-PSMA7 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PSMA7 MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 5 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. [antibody concentration 5 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol