H00005688-M01

antibody from Novus Biologicals

Targeting: PSMA7 C6, HSPC, RC6-1, XAPC7

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005688-M01

Provider

Novus Biologicals

Proper citation

Novus Cat#H00005688-M01, RRID:AB_547394

Product name

Mouse Monoclonal PSMA7 Antibody

Antibody type

Monoclonal

Description

IgG purified. PSMA7 - proteasome (prosome, macropain) subunit, alpha type, 7

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

PSMA7 inhibits the tumorigenicity of A549 human lung adenocarcinoma cells.

Tan JY, Huang X, Luo YL
Molecular and cellular biochemistry 2012 Jul;366(1-2):131-7

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Proteomic identification of putative biomarkers of radiotherapy resistance: a possible role for the 26S proteasome?

Smith L, Qutob O, Watson MB, Beavis AW, Potts D, Welham KJ, Garimella V, Lind MJ, Drew PJ, Cawkwell L
Neoplasia (New York, N.Y.) 2009 Nov;11(11):1194-207

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Proteomic identification of putative biomarkers of radiotherapy resistance: a possible role for the 26S proteasome?

Smith L, Qutob O, Watson MB, Beavis AW, Potts D, Welham KJ, Garimella V, Lind MJ, Drew PJ, Cawkwell L
Neoplasia (New York, N.Y.) 2009 Nov;11(11):1194-207

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of PSMA7 expression in transfected 293T cell line by PSMA7 monoclonal antibody (M01), clone 1A10-3G12.Lane 1: PSMA7 transfected lysate (Predicted MW: 27.9 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - PSMA7 monoclonal antibody (M01), clone 1A10-3G12. Analysis of PSMA7 expression in human omentum, serous carcinoma.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - PSMA7 monoclonal antibody (M01), clone 1A10-3G12 Analysis of PSMA7 expression in HeLa.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Sandwich ELISA: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Detection limit for recombinant GST tagged PSMA7 is approximately 1ng/ml as a capture antibody.

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of monoclonal antibody to PSMA7 on HeLa cell. Antibody concentration 1 ~ 10 ug/ml.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of PSMA7 transfected lysate using anti-PSMA7 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PSMA7 MaxPab rabbit polyclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. Antibody concentration 5 ug/ml.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human colon tissue. Antibody concentration 5 ug/ml.