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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [2]
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- Product number
- BML-PW8120-0025 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#BML-PW8120-0025, RRID:AB_2052373
- Product name
- Proteasome 20S _4 subunit (human) monoclonal antibody (MCP34)
- Antibody type
- Monoclonal
- Antigen
- Purified from natural sources
- Description
- Partially purified ascites.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- MCP34
- Vial size
- 25 μl
- Storage
- -20°C
Submitted references Dynamics of the degradation of ubiquitinated proteins by proteasomes and autophagy: association with sequestosome 1/p62.
Myeku N, Figueiredo-Pereira ME
The Journal of biological chemistry 2011 Jun 24;286(25):22426-40
The Journal of biological chemistry 2011 Jun 24;286(25):22426-40
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis of 20S Proteasome subunit _4, mAb (MCP34) (Prod. No. BML-PW8120): Lane 1: MW marker, Lane 2: HeLa S100 fraction (Prod. No. BML-SW8750), Lane 3: HeLa cell lysate (Prod. No. ADI-LYC-HL100).
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis: Luminograph of material immunoprecipitated from a HeLa S3 S100 fraction (BML-SW8750) using immobilised BML-PW8335 (lane a) or BML-PW9005 (lane b) after SDS-PAGE followed by blotting onto PVDF and probing with antibody BML-PW8155 to proteasome _ and § subunits. Antibody dilution 1:1000 using ECL procedure (1 min exposure).
