AP32840PU-N

antibody from Acris Antibodies GmbH

Targeting: GRM1 GPRC1A, mGlu1, MGLUR1, PPP1R85

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

AP32840PU-N

Provider

Acris Antibodies GmbH

Product name

anti mGluR1a / GRM1a

Antibody type

Polyclonal

Antigen

Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. Production: These synthetic peptides corresponded to different regions of the metabotropic glutamate receptor type 1 gene product, but are shared between the human (NP_000829.2, NCBI) and mouse (NP_058672.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Reactivity

Human, Mouse

Host

Chicken/Avian

Vial size

1.0 ml

Concentration

0.3 mg/ml of anti-peptide antibody mixture (based on Bradford assay readings using BSA as a standard)

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

mGluR1 staining of cortical ons in an adult transgenic Mouse brain (red satining). Green staining is GFP-immunoreactivity for a subpopulation of cortical neurons.