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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- AGC-006-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- mGluR1 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 25 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain lysate: - 1. Anti-mGluR1 (extracellular) Antibody (#AGC-006), (1:200). 2. Anti-mGluR1 (extracellular) Antibody , preincubated with mGluR1 (extracellular) Blocking Peptide (#BLP-GC006).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysisof mouse brain lysate: - 1. Anti-mGluR1 (extracellular) Antibody (#AGC-006), (1:200). 2. Anti-mGluR1 (extracellular) Antibody , preincubated with mGluR1 (extracellular) Blocking Peptide (#BLP-GC006).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of mGluR1 in rat C6 glioma cells - Cell surface detection of mGluR1 in live intact rat C6 glioma cells using Anti-mGluR1 (extracellular) Antibody (#AGC-006), (1:100), followed by goat- Anti-rabbit-AlexaFluor-555 secondary Antibody (red). Nuclei were stained with Hoechst 33342 (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of mGluR1 in rat C6 glioma cells - Cell surface detection of mGluR1 in live intact rat C6 glioma cells using Anti-mGluR1 (extracellular) Antibody (#AGC-006), (1:100), followed by goat- Anti-rabbit-AlexaFluor-555 secondary Antibody (red). Nuclei were stained with Hoechst 33342 (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of mGluR1 in live intact Jurkat cells: - (black) Unstained cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-mGluR1 (extracellular) Antibody (#AGC-006), (10 µg) + goat- Anti-rabbit-FITC.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of rat brain lysate: - 1. Cell lysate + protein A beads + Anti-mGluR1 (extracellular) Antibody (#AGC-006). 2. Cell lysate + protein A beads + pre-immune rabbit serum.3. Cell lysate. Black arrow indicates the mGluR1 protein (glycosylated form) while the red arrow shows the IgG heavy chain. Immunoblot was performed with the Anti-mGluR1 (extracellular) Antibody .
