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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- NBP1-47266 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-47266, RRID:AB_10010220
- Product name
- Rabbit Polyclonal FYCO1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references [Dual Antiplatelet Therapy in the Perioperative Period - To Continue or Discontinue Treatment?]
Central role of mitofusin 2 in autophagosome-lysosome fusion in cardiomyocytes.
Koscielny J, von Heymann C, Zeymer U, Cremer J, Spannagl M, Labenz J, Giannitsis E, Goss F
Deutsche medizinische Wochenschrift (1946) 2017 Aug;142(16):1223-1230
Deutsche medizinische Wochenschrift (1946) 2017 Aug;142(16):1223-1230
Central role of mitofusin 2 in autophagosome-lysosome fusion in cardiomyocytes.
Zhao T, Huang X, Han L, Wang X, Cheng H, Zhao Y, Chen Q, Chen J, Cheng H, Xiao R, Zheng M
The Journal of biological chemistry 2012 Jul 6;287(28):23615-25
The Journal of biological chemistry 2012 Jul 6;287(28):23615-25
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FYCO1 Antibody [NBP1-47266] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-FYCO1 antibody NBP1-47266 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 10 ug/mg lysate. FYCO1 was also immunoprecipitated by rabbit anti-FYCO1 antibody NBP1-47265, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
