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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB11325 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB11325, RRID:AB_1714010
- Product name
- Sipa1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of Sipa1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Bromodomain protein Brd4 binds to GTPase-activating SPA-1, modulating its activity and subcellular localization.
Rap1 GTPase-activating protein SPA-1 negatively regulates cell adhesion.
Human SPA-1 gene product selectively expressed in lymphoid tissues is a specific GTPase-activating protein for Rap1 and Rap2. Segregate expression profiles from a rap1GAP gene product.
Farina A, Hattori M, Qin J, Nakatani Y, Minato N, Ozato K
Molecular and cellular biology 2004 Oct;24(20):9059-69
Molecular and cellular biology 2004 Oct;24(20):9059-69
Rap1 GTPase-activating protein SPA-1 negatively regulates cell adhesion.
Tsukamoto N, Hattori M, Yang H, Bos JL, Minato N
The Journal of biological chemistry 1999 Jun 25;274(26):18463-9
The Journal of biological chemistry 1999 Jun 25;274(26):18463-9
Human SPA-1 gene product selectively expressed in lymphoid tissues is a specific GTPase-activating protein for Rap1 and Rap2. Segregate expression profiles from a rap1GAP gene product.
Kurachi H, Wada Y, Tsukamoto N, Maeda M, Kubota H, Hattori M, Iwai K, Minato N
The Journal of biological chemistry 1997 Oct 31;272(44):28081-8
The Journal of biological chemistry 1997 Oct 31;272(44):28081-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using Sipa1 polyclonal antibody (Cat # PAB11325) shows detection of over-expressed Sipa1 in lysates from mouse NIH/3T3 cells transfected with Sipa1 (Lane 1).Endogenous Sipa1 is detected in lane 2, which contains lysate from NIH/3T3 cells mock-transfected with Lac ZGLB, although at a significantly reduced level compared to transfected cells.Lane 3 and 4 are similar to lanes 1 and 2 except the antibody was preincubated with the immunizing peptide prior to reaction with the membrane.The identity of the higher and lower molecular weight bands is unknown.The band at ~130 KDa, indicated by the arrowhead, corresponds to recombinant Sipa1.The primary antibody was used at 1 : 1250.Personal communication, H. Yang, L. Lukes and K. Hunter, NCI, Bethesda, MD.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry of Sipa1 polyclonal antibody (Cat # PAB11325) was used at 1.25 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.This image shows moderate to strong positive staining of lymphocytes within human tonsil at 40X.Tissue was formalin-fixed and paraffin embedded.The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
