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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-28122 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SIPA1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: SIPA1 tansfected 293T cell.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SIPA1 was performed by separating of whole cell lysates by 7.5% SDS PAGE. Proteins were transferred to a membrane and probed with a SIPA1 Polyclonal Antibody (Product # PA5-28122) at a dilution of 1:500. A: 293T cell line non-transfected lysate. , B: 293T cell line SIPA1 transfected lysate. .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SIPA1 in methanol-fixed mouse ESC cells using a SIPA1 polyclonal antibody (Product # PA5-28122) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SIPA1 Polyclonal Antibody detects SIPA1 protein at weak membrane and cytosol on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. SIPA1 Polyclonal Antibody (Product # PA5-28122) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SIPA1 Polyclonal Antibody detects SIPA1 protein at weak membrane and cytosol on HBL435 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL435 xenograft. SIPA1 Polyclonal Antibody (Product # PA5-28122) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
