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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 690702 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#690702, RRID:AB_2629824
- Product name
- Purified anti-SH2D1A (SAP)
- Antibody type
- Monoclonal
- Description
- SH2D1A, also known as SAP, is a small adaptor protein which plays a major role in the bidirectional stimulation of T and B cells. SH2D1A is predominantly expressed in T cells and NK cells, and functions to regulate signal transduction pathways downstream of the SLAM family of surface receptors. The SH2D1A gene is frequently mutated in patients with X-linked lymphoproliferative disease. Malfunction of the SLAM-SAP pathway results in autoimmune diseases, tumors of the lymphoid tissues and inadequate responses to infectious agents.
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antibody clone number
- 1A9
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell lysates (15 µg protein) from Jurkat (lane 1), mouse thymus (lane 2) and HeLa (lane 3, negative control) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-SH2D1A (clone 1A9) antibody. Proteins were visualized using a goat anti-rat-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Purified anti-GAPDH (clone Poly6314) antibody was used as a loading control.
- Conjugate
- Unconjugated
