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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX125971 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX125971, RRID:AB_11166967
- Product name
- ARD1A antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Reducing Tumour Hypoxia via Oral Administration of Oxygen Nanobubbles.
Owen J, McEwan C, Nesbitt H, Bovornchutichai P, Averre R, Borden M, McHale AP, Callan JF, Stride E
PloS one 2016;11(12):e0168088
PloS one 2016;11(12):e0168088
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: H1299 transfected scramble siRNA B: H1299 transfected ARDIA siRNA 12% SDS PAGE GTX125971 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 D: C2C12 E: Raw264.7 12% SDS PAGE GTX125971 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ARD1A antibody detects ARD1A protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and blotted with ARD1A antibody (GTX125971) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ARD1A antibody detects NAA10 protein at Cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in -20¢J 100% MeOH for 5 min.Green: NAA10 protein stained by ARD1A antibody (GTX125971) diluted at 1:500.Blue: Hoechst 33343 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of ARD1A protein from HeLa whole cell extracts using 5 £gg of ARD1A antibody (GTX125971).Western blot analysis was performed using ARD1A antibody (GTX125971).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using ARD1A(GTX125971) antibody at 1:500 dilution.
