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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PA5-116361 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SEMA4G Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western Blot analysis of SEMA4G using the SEMA4G Polyclonal Antibody (Product # PA5-116361) at a 1:2000 dilution. Lane 1: A549 whole cell lysates, Lane 2: Hela whole cell lysates, Lane 3: HT-29 whole cell lysates, Lane 4: SH-SY5Y whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution Predicted band size : 91 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
