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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- ABIN501071 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Tuberous Sclerosis 1 (TSC1) (Middle Region), (both Isoforms) antibody
- Antibody type
- Polyclonal
- Antigen
- TSC1 antibody was raised against a 15 amino acid peptide from the middle region of human TSC1. TSC1 antibody will recognize both isoforms of TSC1.
- Description
- Peptide affinity chromatography
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Epitope
- Middle Region,both Isoforms
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store at 2 - 8°C for up to one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Signaling by target of rapamycin proteins in cell growth control.
Identification of dominant negative mutants of Rheb GTPase and their use to implicate the involvement of human Rheb in the activation of p70S6K.
Integration of growth factor and nutrient signaling: implications for cancer biology.
Rheb GTPase is a direct target of TSC2 GAP activity and regulates mTOR signaling.
Inoki K, Ouyang H, Li Y, Guan KL
Microbiology and molecular biology reviews : MMBR 2005 Mar;69(1):79-100
Microbiology and molecular biology reviews : MMBR 2005 Mar;69(1):79-100
Identification of dominant negative mutants of Rheb GTPase and their use to implicate the involvement of human Rheb in the activation of p70S6K.
Tabancay AP Jr, Gau CL, Machado IM, Uhlmann EJ, Gutmann DH, Guo L, Tamanoi F
The Journal of biological chemistry 2003 Oct 10;278(41):39921-30
The Journal of biological chemistry 2003 Oct 10;278(41):39921-30
Integration of growth factor and nutrient signaling: implications for cancer biology.
Shamji AF, Nghiem P, Schreiber SL
Molecular cell 2003 Aug;12(2):271-80
Molecular cell 2003 Aug;12(2):271-80
Rheb GTPase is a direct target of TSC2 GAP activity and regulates mTOR signaling.
Inoki K, Li Y, Xu T, Guan KL
Genes & development 2003 Aug 1;17(15):1829-34
Genes & development 2003 Aug 1;17(15):1829-34
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of TSC1 in EL4 cell lysate with AP30960PU-N at 1 ?g/ml in the (A) absence and (B) presence of blocking peptide.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunocytochemistry of TSC1 in EL4 cells with AP30960PU-N at 2 ?g/ml
