11170-1-AP

antibody from Invitrogen Antibodies

Targeting: EIF2S1 EIF-2alpha, EIF2, EIF2A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11170-1-AP

Provider

Invitrogen Antibodies

Product name

EIF2S1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MPGLSCRFY QHKFPEVEDV VMVNVRSIAE MGAYVSLLEY NNIEGMILLS ELSRRRIRSI NKLIRIGRNE CVVVIRVDKE KGYIDLSKRR VSPEEAIKCE DKFTKSKTVY SILRHVAEVL EYTKDEQLES LFQRTAWVFD DKYKRPGYGA YDAFKHAVSD PSILDSLDLN EDEREVLINN INRRLTPQAV KIRADIEVAC YGYEGIDAVK EALRAGLNCS TENMPIKINL IAPPRYVMTT TTLERTEGLS VLSQAMAVIK EKIEEKRGVF NVQMEPKVVT DTDETELARQ MERLERENAE VDGDDDAEEM EAKAED (1-315 aa encoded by B C002513)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.27 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human stomach tissue were subjected to SDS PAGE followed by western blot with 11170-1-AP (EIF2S1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human placenta tissue were subjected to SDS PAGE followed by western blot with 11170-1-AP (EIF2S1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11170-1-AP (EIF2S1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using EIF2S1 antibody 11170-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 11170-1-AP (EIF2S1 antibody) at dilution of 1:50 (under 10x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HeLa cells were stained with 0.2 ug EIF2S1 antibody (11170-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) with dilution 1:1500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-EIF2S1 (IP:11170-1-AP, 4ug; Detection:11170-1-AP 1:1000) with HepG2 cells lysate 2400ug.