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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB4412 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4412, RRID:AB_1576704
- Product name
- KLK6 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of KLK6.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Human kallikrein 6 activity is regulated via an autoproteolytic mechanism of activation/inactivation.
Cloning and characterization of novel isoforms of the human kallikrein 6 gene.
Distinct promoters regulate tissue-specific and differential expression of kallikrein 6 in CNS demyelinating disease.
Bayés A, Tsetsenis T, Ventura S, Vendrell J, Aviles FX, Sotiropoulou G
Biological chemistry 2004 Jun;385(6):517-24
Biological chemistry 2004 Jun;385(6):517-24
Cloning and characterization of novel isoforms of the human kallikrein 6 gene.
Pampalakis G, Kurlender L, Diamandis EP, Sotiropoulou G
Biochemical and biophysical research communications 2004 Jul 16;320(1):54-61
Biochemical and biophysical research communications 2004 Jul 16;320(1):54-61
Distinct promoters regulate tissue-specific and differential expression of kallikrein 6 in CNS demyelinating disease.
Christophi GP, Isackson PJ, Blaber S, Blaber M, Rodriguez M, Scarisbrick IA
Journal of neurochemistry 2004 Dec;91(6):1439-49
Journal of neurochemistry 2004 Dec;91(6):1439-49
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of KLK6 (arrow) using KLK6 polyclonal antibody (Cat # PAB4412).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KLK6 gene (Lane 2).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer was performed with KLK6 polyclonal antibody (Cat # PAB4412) at 1:10-1:50 dilution followed by indirect peroxidase conjugation with secondary antibody and DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of 293 cells (right histogram), compared to a negative control cell (left histogram), was performed with KLK6 polyclonal antibody (Cat # PAB4412) and FITC-conjugated goat-anti-rabbit secondary antibody.
- Validation comment
- Flow Cytometry
