Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Other assay [1]
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Validation data
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- Product number
- 21548-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UBE4A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: QLAEALIKV FVDIEFTGDP HQFEQKFNYR RPMYPILRYM WGTDTYRESI KDLADYASKN LEAMNPPLFL RFLNLLMNDA IFLLDEAIQY LSKIKIQQIE KDRGEWDSLT PEARREKEAG LQMFGQLARF HNIMSNETIG TLAFLTSEIK SLFVHPFLAE RIISMLNYFL QHLVGPKMGA LKVKDFSEFD FKPQQLVSDI CTIYLNLGDE ENFCATVPKD GRSYSPTLFA QTVRVLKKIN KPGNMIMAFS NLAERIKSLA DLQQQEEETY ADACDEFLDP IMSTLMCDPV VLPSSRVTVD RSTIARHLLS DQTDPFNRSP LTMDQIRPNT ELKEKIQRWL AERKQQKEQL E (717-1066 aa encoded by BC112367)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.21 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 21548-1-AP (UBE4A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 21548-1-AP (UBE4A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 21548-1-AP (UBE4A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP Result of anti-UBE4A (Product # 21548-1-AP) with mouse brain tissue lysate 4,000 µg. IP: 21548-1-AP, 4 µg; Detection: 21548-1-AP 1:500.