A010-14

antibody from Badrilla Ltd
Targeting: PLN CMD1P, PLB
Provider product page for A010-14
Western blot
Immunohistochemistry

Antibody data

Product number
A010-14 - Provider product page
Provider
Badrilla Ltd
Proper citation
Badrilla Cat#A010-14, RRID:AB_2617049
Product name
Phospholamban A1 Antibody (IgG)
Antibody type
Monoclonal
Antigen
Purifed from natural sources
Description
The majority of calcium required for muscle contraction is mobilised from the Sarcoplasmic Reticulum (SR), to which it has to return to in order to facilitate relaxation. The (Ca2+- Mg2+) ATPase enzyme is responsible for the sequestration of calcium after its release from the SR, and is controlled by another sarcoplasmic reticulum protein called phospholamban (PLB) in cardiac, smooth and slow-twitch skeletal muscle. The monoclonal antibody (A1) was raised against bovine cardiac PLB and recognises an epitope comprising of residues 7LTRSAIRRAS16. The antibody reacts with all forms of PLB: monomer, oligomers, phosphorylated and non-phosphorylated. The antibody stimulates Ca2+ pump activity upon binding to PLB, to an extent equivalent to stoichiometric phosphorylation of PLB Manipulation of SERCA2:PLB ratios is a therapeutic strategy for heart failure. Elimination of PLB boosts SR Ca2+-load which can exacerbate pathological outcomes (apoptosis, arrhythmias) when combined with other alterations in SR function.
Host
Mouse
Antigen sequence
bovine phospholamban
Isotype
IgG
Vial size
20 µl
Storage
Lyophilised: 4 °C Reconstituted:-80 °C
Handling
Reconstitute lyophilised powder in 20 µl of 18 MΩ H2O, aliquot and store frozen at -80 °C for 1 year. Avoid freeze - thaw cycles
PLN protein structure - A010-14 shown in red.