11749-1-AP

antibody from Invitrogen Antibodies

Targeting: PSMB1 HC5, PMSB1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

11749-1-AP

Provider

Invitrogen Antibodies

Product name

PSMB1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MLSSTAMYS APGRDLGMEP HRAAGPLQLR FSPYVFNGGT ILAIAGEDFA IVASDTRLSE GFSIHTRDSP KCYKLTDKTV IGCSGFHGDC LTLTKIIEAR LKMYKHSNNK AMTTGAIAAM LSTILYSRRF FPYYVYNIIG GLDEEGKGAV YSFDPVGSYQ RDSFKAGGSA SAMLQPLLDN QVGFKNMQNV EHVPLSLDRA MRLVKDVFIS AAERDVYTGD ALRICIVTKE GIREETVSLR KD (1-241 aa encoded by BC020807)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.3 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 11749-1-AP (PSMB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 11749-1-AP (PSMB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using (Product # 11749-1-AP) (PSMB1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red stain with CL594-Phalloidin. Blue stain with DAPI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using PSMB1 antibody 11749-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 11749-1-AP (PSMB1 antibody) at dilution of 1:50 (under 10x lens).