Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- AF2104 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TSG-6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human TSG-6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 70% cross-reactivity with recombinant mouse TSG-6 is observed and less than 2% cross-reactivity with recombinant human TSG-14 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P98066
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Concentrated Conditioned Media from Adipose Tissue Derived Mesenchymal Stem Cells Mitigates Visual Deficits and Retinal Inflammation Following Mild Traumatic Brain Injury.
Jha KA, Pentecost M, Lenin R, Klaic L, Elshaer SL, Gentry J, Russell JM, Beland A, Reiner A, Jotterand V, Sohl N, Gangaraju R
International journal of molecular sciences 2018 Jul 11;19(7)
International journal of molecular sciences 2018 Jul 11;19(7)
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- TSG-6 in human PBMCs. TSG-6 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human TSG-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2104) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.