NB200-208

antibody from Novus Biologicals

Targeting: KDR CD309, FLK1, VEGFR, VEGFR2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB200-208

Provider

Novus Biologicals

Proper citation

Novus Cat#NB200-208, RRID:AB_10000504

Product name

Mouse Monoclonal VEGFR2/KDR/Flk-1 Antibody

Antibody type

Monoclonal

Description

Protein G purified. The monoclonal will detect native human VEGFR-2/ KDR in ELISA experiments and on the surface of different human cell types.

Reactivity

Human, Rat

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

LYOPH

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Stromal cell-derived factor-1alpha activation of tissue-engineered endothelial progenitor cell matrix enhances ventricular function after myocardial infarction by inducing neovasculogenesis.

Frederick JR, Fitzpatrick JR 3rd, McCormick RC, Harris DA, Kim AY, Muenzer JR, Marotta N, Smith MJ, Cohen JE, Hiesinger W, Atluri P, Woo YJ
Circulation 2010 Sep 14;122(11 Suppl):S107-17

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Acute myocardial rescue with endogenous endothelial progenitor cell therapy.

Atluri P, Panlilio CM, Liao GP, Hiesinger W, Harris DA, McCormick RC, Cohen JE, Jin T, Feng W, Levit RD, Dong N, Woo YJ
Heart, lung & circulation 2010 Nov;19(11):644-54

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: VEGF R2/KDR/Flk-1 Antibody (EIC) [NB200-208] - Analysis of anti-human VEGFR-2/KDR. Samples were loaded in 10% SDS-polyacrylamide gel under reducing conditions.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: VEGF R2/KDR/Flk-1 Antibody (EIC) [NB200-208] - Analysis using the Biotin conjugate of NB200-208. Detection of anti-human VEGFR-2/KDR. Samples were loaded in 10% SDS-polyacrylamide gel under reducing conditions.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: VEGF R2/KDR/Flk-1 Antibody (EIC) [NB200-208] - Functional ELISA with anti-human VEGFR-2/KDR. Recombinant human soluble KDR (D7) was coated with increasing amounts in a 96 well microtiter plate. The antibody was used at 1ug/ml.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: VEGF R2/KDR/Flk-1 Antibody (EIC) [NB200-208] - Analysis of VEGFR-2/KDR expression in HUVE cells (upper level) and EPCs derived from PBMcs (lower level ) [5ug/ml]; 5ug/ml PE goat anti-mouse IgG]. Image provided by Trisha M. Westerhof, University of California, Irvine.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: VEGF R2/KDR/Flk-1 Antibody (EIC) [NB200-208] - Analysis using the Biotin conjugate of NB200-208. Staining of VEGFR-2/KDR expression in HUVE cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: VEGF R2/KDR/Flk-1 Antibody (EIC) [NB200-208] - Flow Cytometry: VEGF R2/KDR/Flk-1 Antibody (EIC) [Allophycocyanin] [NB200-208APC] - HUVECs were unstained (left) or stained (right) with VEGFR-2/KDR. Image from verified customer review. Image using the Allophycocyanin form of this antibody.