R1041X

antibody from OriGene

Targeting: COL4A1

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R1041X

Provider

OriGene

Product name

Collagen IV (COL4A1) rabbit polyclonal antibody, Aff - Purified

Antibody type

Polyclonal

Description

Collagen IV (COL4A1) rabbit polyclonal antibody, Aff - Purified

Host

Rabbit

Conjugate

Unconjugated

Epitope

COL4A1

Antibody clone number

NULL

Vial size

500 µg

Concentration

1.0 mg/ml (by UV absorbance at 280 nm)

References

Submitted references

Perlecan is critical for heart stability.

Sasse P, Malan D, Fleischmann M, Roell W, Gustafsson E, Bostani T, Fan Y, Kolbe T, Breitbach M, Addicks K, Welz A, Brem G, Hescheler J, Aszodi A, Costell M, Bloch W, Fleischmann BK
Cardiovascular research 2008 Dec 1;80(3):435-44

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

R1041 Collagen IV antibody (1/400, 45 min RT) showed strong staining in FFPE sections of Human kidney (Left) with strong Red staining observed in glomeruli and liver (Right) with strong staining in sinusoids. Staining for both tissues was consistent with a basement membrane distribution. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WA

Validation comment

IHC