NBP1-26549

antibody from Novus Biologicals

Targeting: COL4A1

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-26549

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-26549, RRID:AB_1853202

Product name

Goat Polyclonal Collagen IV alpha 1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Reacts with conformational determinants on human type IV collagen as demonstrated by ELISA. May react with type IV collagen from other species. Exhibits

Reactivity

Human, Mouse, Rat, Bovine, Simian

Host

Goat

Isotype

IgG

Vial size

0.1 mg

Concentration

0.4 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

Optimal Mass Transport with Lagrangian Workflow Reveals Advective and Diffusion Driven Solute Transport in the Glymphatic System.

Koundal S, Elkin R, Nadeem S, Xue Y, Constantinou S, Sanggaard S, Liu X, Monte B, Xu F, Van Nostrand W, Nedergaard M, Lee H, Wardlaw J, Benveniste H, Tannenbaum A
Scientific reports 2020 Feb 6;10(1):1990

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A type IV collagenase inhibitor, N-hydroxy-3-phenyl-2-(4-phenylbenzenesulfonamido) propanamide (BiPS), suppresses skin injury induced by sulfur mustard.

Chang YC, Hahn RA, Gordon MK, Laskin JD, Gerecke DR
Toxicology and applied pharmacology 2020 Aug 15;401:115078

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A Fully Automated High-Throughput Bioreactor System for Lung Regeneration.

Gorman DE, Wu T, Gilpin SE, Ott HC
Tissue engineering. Part C, Methods 2018 Nov;24(11):671-678

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Polidocanol induced tubal occlusion in nonhuman primates: immunohistochemical detection of collagen I-V.

Slayden OD, Lee DO, Yao S, Jensen JT
Contraception 2016 Nov;94(5):521-526

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Collagen IV alpha 1 Antibody [NBP1-26549] - Human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (left) and Goat Anti-Type IV Collagen-UNLB; (right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP DAB, and hematoxylin.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: Collagen IV alpha 1 Antibody [NBP1-26549] - Frozen sections of E12.5 eyes from Col4a1+/+ mice were stained with Goat Anti-Type IV Collagen-UNLB followed by a secondary antibody and DAPI. Image from Mao M, Kiss M, Ou Y, Gould DB. Genetic dissection of anterior segment dysgenesis caused by a Col4a1 mutation. Dis Model Mech. 2017 Feb 24. doi: 10.1242/dmm.027888.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: Collagen IV alpha 1 Antibody [NBP1-26549] - Frozen Itga3-/-/Gpr56-/- E11.5 mouse neocortex section was stained with Goat Anti-Type IV Collagen-UNLB anti-Tuj1, and anti-Nestin followed by secondary antibodies. Image from Jeong S, Luo R, Singer K, Giera S, Kreidberg J, Kiyozumi D, et al. GPR56 functions together with alpha 3beta1 integrin in regulating cerebral cortical development. PLoS One. 2013;8(7):e68781.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: Collagen IV alpha 1 Antibody [NBP1-26549] - Frozen rat kidney section following SNX was stained with Goat Anti-Type IV Collagen-UNLB followed by a secondary antibody and DAB. Image from Yuen DA, Connelly KA, Advani A, Liao C, Kuliszewski MA, Trogadis J, et al. Culture-modified bone marrow cells attenuate cardiac and renal injury in a chronic kidney disease rat model via a novel antifibrotic mechanism. PLoS One. 2010;5(3):e9543.