362701

antibody from BioLegend

Targeting: CD3E

Western blot (Data presented on provider website)

Immunocytochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

362701

Provider

BioLegend

Proper citation

BioLegend Cat#362701, RRID:AB_2563713

Product name

Purified anti-human/mouse CD3ε (activation epitope)

Antibody type

Monoclonal

Antigen

Purified denatured CD3 proteins from human thymus

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

APA1/1

Vial size

50 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood lymphocytes were fixed and permeabilized with 70% ethanol, followed by intracellular staining with purified CD3e (clone APA1/1, filled histogram) or mouse IgG1, κ isotype control (open histogram) followed by anti-mouse IgG PE.

Conjugate

Unconjugated