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Immunohistochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 317303 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#317303, RRID:AB_571924
- Product name
- LEAF™ Purified anti-human CD3 [OKT3]
- Antibody type
- Monoclonal
- Antigen
- CD3
- Description
- CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 1 mg/ml
- Storage
- The CD3 antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Submitted references F-actin polymerization and retrograde flow drive sustained PLCĪ³1 signaling during T cell activation.
High-resolution imaging of the immunological synapse and T-cell receptor microclustering through microfabricated substrates.
Loss of Kindlin-3 in LAD-III eliminates LFA-1 but not VLA-4 adhesiveness developed under shear flow conditions.
Ligand-independent exhaustion of killer immunoglobulin-like receptor-positive CD8+ T cells in human immunodeficiency virus type 1 infection.
Blocking of monocyte-associated B7-H1 (CD274) enhances HCV-specific T cell immunity in chronic hepatitis C infection.
Babich A, Li S, O'Connor RS, Milone MC, Freedman BD, Burkhardt JK
The Journal of cell biology 2012 Jun 11;197(6):775-87
The Journal of cell biology 2012 Jun 11;197(6):775-87
High-resolution imaging of the immunological synapse and T-cell receptor microclustering through microfabricated substrates.
Biggs MJ, Milone MC, Santos LC, Gondarenko A, Wind SJ
Journal of the Royal Society, Interface / the Royal Society 2011 Oct 7;8(63):1462-71
Journal of the Royal Society, Interface / the Royal Society 2011 Oct 7;8(63):1462-71
Loss of Kindlin-3 in LAD-III eliminates LFA-1 but not VLA-4 adhesiveness developed under shear flow conditions.
Manevich-Mendelson E, Feigelson SW, Pasvolsky R, Aker M, Grabovsky V, Shulman Z, Kilic SS, Rosenthal-Allieri MA, Ben-Dor S, Mory A, Bernard A, Moser M, Etzioni A, Alon R
Blood 2009 Sep 10;114(11):2344-53
Blood 2009 Sep 10;114(11):2344-53
Ligand-independent exhaustion of killer immunoglobulin-like receptor-positive CD8+ T cells in human immunodeficiency virus type 1 infection.
Alter G, Rihn S, Streeck H, Teigen N, Piechocka-Trocha A, Moss K, Cohen K, Meier A, Pereyra F, Walker B, Altfeld M
Journal of virology 2008 Oct;82(19):9668-77
Journal of virology 2008 Oct;82(19):9668-77
Blocking of monocyte-associated B7-H1 (CD274) enhances HCV-specific T cell immunity in chronic hepatitis C infection.
Jeong HY, Lee YJ, Seo SK, Lee SW, Park SJ, Lee JN, Sohn HS, Yao S, Chen L, Choi I
Journal of leukocyte biology 2008 Mar;83(3):755-64
Journal of leukocyte biology 2008 Mar;83(3):755-64
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Human peripheral blood lymphocytes stained with LEAF™ purified OKT3, followed by anti-mouse IgG FITC
- Conjugate
- Unconjugated
