AM12005PU-N

antibody from Acris Antibodies GmbH

Targeting: CD3E

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

AM12005PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM12005PU-N, RRID:AB_10554868

Product name

anti CD3 (activation epitope)

Antibody type

Monoclonal

Antigen

Purified human CD3 proteins isolated from thymus

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

APA1/1

Vial size

0.1 mg

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Jurkat cell lysate was probed with anti-CD3 epsilon AM12005PU-N (0.5μg/ml).

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

T-cell Activation by an Antigen presenting Cell. Panel A is an image of a single Antigen-Presenting Cell (APC; a Raji cell, stained blue with CMAC dye) activated by Staphylococcal enterotoxin E in contact with a single T-cell (a Jurkat cell). Panel B is an image of the two cells, stained with an anti-CD3ζ antiserum (a 1:3,000 dilution) and anti-CD3ε, clone APA1/1 (Cat. #AM12005PU-N; 10µg/ml). Visualization of CD3ζ or CD3ε was with an anti-rabbit Alexa 488 (green) or anti-mouse Alexa 594 (red) conjugate, respectively; the yellow color is produced by co-localization of CD3ζ and CD3ε (clone APA1/1) staining. Data generously provided by Dr. Balbino Alarcon, Centro de Biologia Molecular, CSIC, Madrid, Spain.