- GTX102965 - GeneTex TP53 antibody

Submitted references Nano-Diamino-Tetrac (NDAT) Enhances Resveratrol-Induced Antiproliferation by Action on the RRM2 Pathway in Colorectal Cancers.

Nana AW, Wu SY, Yang YS, Chin YT, Cheng TM, Ho Y, Li WS, Liao YM, Chen YR, Shih YJ, Liu YR, Pedersen J, Incerpi S, Hercbergs A, Liu LF, Whang-Peng J, Davis PJ, Lin HY
Hormones & cancer 2018 Oct;9(5):349-360

Systematic discovery of drug action mechanisms by an integrated chemical genomics approach: identification of functional disparities between azacytidine and decitabine.

Hsieh YY, Huang TC, Lo HL, Jhan JY, Chen ST, Yang PM
Oncotarget 2016 May 10;7(19):27363-78

miR-494-3p Induces Cellular Senescence and Enhances Radiosensitivity in Human Oral Squamous Carcinoma Cells.

Weng JH, Yu CC, Lee YC, Lin CW, Chang WW, Kuo YL
International journal of molecular sciences 2016 Jul 8;17(7)

Simvastatin induced HCT116 colorectal cancer cell apoptosis through p38MAPK-p53-survivin signaling cascade.

Chang HL, Chen CY, Hsu YF, Kuo WS, Ou G, Chiu PT, Huang YH, Hsu MJ
Biochimica et biophysica acta 2013 Aug;1830(8):4053-64

Human parvovirus B19 DNA replication induces a DNA damage response that is dispensable for cell cycle arrest at phase G2/M.

Lou S, Luo Y, Cheng F, Huang Q, Shen W, Kleiboeker S, Tisdale JF, Liu Z, Qiu J
Journal of virology 2012 Oct;86(19):10748-58

Citrullination of inhibitor of growth 4 (ING4) by peptidylarginine deminase 4 (PAD4) disrupts the interaction between ING4 and p53.

Guo Q, Fast W
The Journal of biological chemistry 2011 May 13;286(19):17069-78

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Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) HCT116 whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody (GTX102965) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
KD Reagent Type: siRNA
Downregulation: >50%
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
KD Reagent Prod page: KD Reagent Prod page
Antibody Lot Number: 41983

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ?g of whole cell lysate) A: HCT116 cells with mock treatment for 24 hr B: HCT116 cells with 30 ?M cisplatin treatment for 24 hr 10% SDS PAGE GTX102965 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ?g of whole cell lysate) A: A431 (GTX27909) 10% SDS PAGE GTX102965 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) HCT116 whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody (GTX102965) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using p53(GTX102965) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: p53 antibody detects p53 protein at nucleus by immunofluorescent analysis. Samples: HCT 116 cells mock (left) and treated with 30 £gM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 protein stained by p53 antibody (GTX102965) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-p53 antibody (GTX102965).?(A) Input, 20ug of HCT116 lysates. The precipitated protein was detected by GTX102965 diluted at 1:10000. EasyBlot anti-Rabbit IgG Kit (GTX225856-01) was used in Western blot.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using p53(GTX102965) antibody at 1:500 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: p53 antibody immunoprecipitates p53 protein-DNA complex in ChIP experiments. ChIP Sample: HCT116 whole cell lysate/extract treated with CPT 500nM for 6hr A. 5 £gg preimmune rabbit IgGB. 5 £gg of p53 antibody (GTX102965)The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.

GTX102965

Antibody data