NB200-104

antibody from Novus Biologicals

Targeting: TP53 LFS1, p53

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Radioimmunoassay (Data presented on provider website)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NB200-104

Provider

Novus Biologicals

Proper citation

Novus Cat#NB200-104, RRID:AB_10001307

Product name

Mouse Monoclonal p53 Antibody

Antibody type

Monoclonal

Description

Protein G purified. Recognizes both wild-type and mutant forms of human p53 protein.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ul

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Identification and comparative analyses of myocardial miRNAs involved in the fetal response to maternal obesity.

Maloyan A, Muralimanoharan S, Huffman S, Cox LA, Nathanielsz PW, Myatt L, Nijland MJ
Physiological genomics 2013 Oct 1;45(19):889-900

---

Oncogenic functions of secreted Frizzled-related protein 2 in human renal cancer.

Yamamura S, Kawakami K, Hirata H, Ueno K, Saini S, Majid S, Dahiya R
Molecular cancer therapeutics 2010 Jun;9(6):1680-7

---

COP1, the negative regulator of p53, is overexpressed in breast and ovarian adenocarcinomas.

Dornan D, Bheddah S, Newton K, Ince W, Frantz GD, Dowd P, Koeppen H, Dixit VM, French DM
Cancer research 2004 Oct 15;64(20):7226-30

---

Immunohistochemical analysis of p53 expression in malignant lymphomas.

Said JW, Barrera R, Shintaku IP, Nakamura H, Koeffler HP
The American journal of pathology 1992 Dec;141(6):1343-8

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: p53 Antibody (PAb 1801) [NB200-104] - Lanes are as follows:. Lane 1 - 20ug of A549 lysate, lane 2 - 10ug of A549 lysate, lane 3 - 5ug of A549 lysate, lane 4 - 2.5ug of A549 lysate, lane 5 - 1.5ug of A549 lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: p53 Antibody (Pab1801) [NB200-104] - Sample (30 ug of whole cell lysate) A: HCT116 cells with mock treatment for 24hr B: HCT116 cells with 30uM cisplatin treatment for 24hr 10% SDS PAGE.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: p53 Antibody (Pab1801) [NB200-104] - Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal mouse IgG or (C) 2.5 ug of anti-p53 antibody (NB200-104). (A) Input, 20ug of HCT116 lysate. The precipitated protein was detected by NB200-104. EasyBlot anti-Mouse IgG Kit was used in Western blot.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: p53 Antibody (PAb 1801) [NB200-104] - Tumor tissues from human medulloblastoma cells xenografted in the mouse cerebellum stained with p53 antibody. Image from verified customer review.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: p53 Antibody (PAb 1801) [NB200-104] - Paraffin-embedded NCl-H1299 xenograft. p53 antibody [Pab1801]) diluted at 1:200.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Chromatin Immunoprecipitation: p53 Antibody (Pab1801) [NB200-104] - Immunoprecipitates p53 protein-DNA complex in ChIP experiments. ChIP Sample: HCT116 whole cell Lysate/extract treated with CPT 500nM for 6hr A. 5 ug preimmune mouse IgG B. 5 ug of p53 antibody (NB200-104) The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.