PA5-117846

antibody from Invitrogen Antibodies

Targeting: CCR3 CC-CKR-3, CD193, CKR3, CMKBR3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117846

Provider

Invitrogen Antibodies

Product name

CCR3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot of CCR3 in Lane A: Daudi Whole Cell lysate, Lane B: K562 Whole Cell Lysate. Samples (30 µg per lane) were incubated with polyclonal antibody (Product # PA5-117846) with a dilution of 1:500 , followed by Goat Anti-Rabbit IgG H&L (Dylight 800) using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 43 kDa, Observed band size: 62 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CCR3 Polyclonal Antibody (Product # PA5-117846) at 1:500 dilution. Lane A: Daudi Whole Cell Lysate, Lane B: K562 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti- RabbitIgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 62 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of CCR3 in 293H cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with CCR3 Polyclonal Antibody (Product # PA5-117846, 1:5,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of CCR3 in Lane A: 0.5 mg Jurkat Whole Cell Lysate. Samples were treated with 60 μg of immunomagnetic Protein G beads, incubated with polyclonal antibody (Product # PA5-117846) with a dilution of 1:500, followed by Dylight 800-labeled antibody to rabbit IgG (H+L) using a dilution of 1:5,000. Assay was performed under reducing conditions. Predicted band size: 43 kDa, Observed band size: 62 kDa .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CCR3 Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 1 µL with CCR3 Polyclonal Antibody (Product # PA5-117846) and 60 μg of Immunomagnetic beads Protein G. Primary antibody: CCR3 Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 62 kDa.