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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- E35-365R - Provider product page
- Provider
- SignalChem
- Product name
- Anti-phospho-4EBP1 (Thr45)
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Purified by affinity chromatography
- Reactivity
- Human, Mouse, Rat
- Epitope
- Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr45
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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Supportive validation
- Submitted by
- SignalChem (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from MDA435 cells untreated or treated with EGF (200nm, 5min) using 4EBP1 antibody (lanes 1 and 2) and anti-phospho-4EBP1 (Thr45) antibody (lanes 3 and 4).
- Sample type
- Western Blot
Supportive validation
- Submitted by
- SignalChem (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-phospho-4EBP1 (Thr45) antibody.
- Sample type
- Immunohistochemistry
