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LearnA700-055
antibody from Invitrogen Antibodies
Targeting: TNFRSF8
CD30, D1S166E, KI-1
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- A700-055 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD30 Recombinant Rabbit Monoclonal Antibody (BLR055F)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- BLR055F
- Vial size
- 100 µL
- Concentration
- 250 µg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CD30 by western blot. Samples: Whole cell lysate (50 µg) from MJ, SR, C5/MJ, GaMG, U2OS and A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD30 recombinant monoclonal antibody [BLR055F] (Product # A700-055 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CD30 by immunocytochemistry.Sample: FFPE section of human SR cells.Antibody: Rabbit anti-CD30 recombinant monoclonal antibody [BLR055F] (Product # A700-055 lot 1) used at 1:250.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CD30 by immunohistochemistry. AntibodySample: FFPE section of human tonsil.Antibody: Rabbit anti-CD30 recombinant monoclonal antibody (BLR055F) (A700-055 lot 1) used at 1:250.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CD30 (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-CD30 recombinant monoclonal antibody [BLR055F] (Product # A700-055 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: Opal™. Counterstain: DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CD30 (shaded) in MJ[G11) cells by flow cytometry. Antibody: Rabbit anti-CD30 recombinant monoclonal [BLR055F] (Product #A700-055; lot 1) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG (Product # A120-101D2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CD30 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MJ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD30 recombinant monoclonal antibody [BLR055F] (Product # A700-055 lot 1) used for IP at 5 µL per reaction. CD30 was also immunoprecipitated by a rabbit anti-CD30 antibody (BL16009) and rabbit anti-CD30 recombinant monoclonal antibody (BL-757-1D4). For blotting immunoprecipitated CD30 (Product # A700-055) was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.
