MA1-81489

antibody from Invitrogen Antibodies

Targeting: CD59 16.3A5, EJ16, EJ30, EL32, G344, MIC11, MIN1, MIN2, MIN3, MSK21, p18-20

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-81489

Provider

Invitrogen Antibodies

Product name

CD59 Monoclonal Antibody (YTH53.1)

Antibody type

Monoclonal

Antigen

Other

Description

Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. A suggested positive control for immunohistochemical applications is placenta. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Antibody clone number

YTH53.1

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot was performed using Anti-CD59 Monoclonal Antibody (YTH53.1) (Product # MA1-81489) and a 21 kDa band corresponding to CD59 glycoprotein was observed across all the cell lines and tissue lysates tested. Membrane enriched extracts (30 µg lysate) of MDA-MB-231 (Lane 1), SK-BR-3 (Lane 2), T-47D (Lane 3), PC-3 (Lane 4), HUVEC (Lane 5), HeLa (Lane 6), A549 (Lane 7), Caco-2 (Lane 8) and Mouse Lung (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with F(ab2-Rabbit anti-Rat IgG (H+L Secondary Antibody, HRP (Product # PA1-29927, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).