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LearnMA1-81489
antibody from Invitrogen Antibodies
Targeting: CD59
16.3A5, EJ16, EJ30, EL32, G344, MIC11, MIN1, MIN2, MIN3, MSK21, p18-20
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- MA1-81489 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD59 Monoclonal Antibody (YTH53.1)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. A suggested positive control for immunohistochemical applications is placenta. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL. Rat anti Human CD59 antibody, clone YTH53.1 recognizes the human CD59 cell surface antigen, also known as MAC-inhibitory protein, 1F5 antigen, 20 kDa homologous restriction factor, MEM43 antigen, membrane attack complex inhibition factor, membrane inhibitor of reactive lysis or protectin.
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- YTH53.1
- Vial size
- 200 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-CD59 Monoclonal Antibody (YTH53.1) (Product # MA1-81489) and a 21 kDa band corresponding to CD59 glycoprotein was observed across all the cell lines and tissue lysates tested. Membrane enriched extracts (30 µg lysate) of MDA-MB-231 (Lane 1), SK-BR-3 (Lane 2), T-47D (Lane 3), PC-3 (Lane 4), HUVEC (Lane 5), HeLa (Lane 6), A549 (Lane 7), Caco-2 (Lane 8) and Mouse Lung (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with F(ab2-Rabbit anti-Rat IgG (H+L Secondary Antibody, HRP (Product # PA1-29927, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
