Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
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Validation data
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- Product number
- LS-C34648 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-C34648, RRID:AB_2159549
- Product name
- PAX5 Antibody (aa2-14) LS-C34648
- Antibody type
- Polyclonal
- Description
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Reactivity
- Human, Simian
- Host
- Goat
- Storage
- Aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- PAX5 antibody staining (3 ug/ml) of U937 lysate (RIPA buffer, 30g total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- PAX5 antibody staining (3 ug/ml) of U937 lysate (RIPA buffer, 30g total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- PAX5 / BSAP antibody (0.3µg/ml) staining of Human Lymph lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- PAX5 / BSAP antibody (1µg/ml) staining of Mouse (A) and (0.3ug/ml) Rat (B) Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- PAX5 / BSAP antibody (0.3µg/ml) staining of Human Lymph lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- PAX5 / BSAP antibody (1µg/ml) staining of Mouse (A) and (0.3ug/ml) Rat (B) Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.