Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00005079-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005079-M01, RRID:AB_546784
- Product name
- Mouse Monoclonal Pax5/BSAP Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PAX5 - paired box gene 5 (B-cell lineage specific activator)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Pax5/BSAP Antibody (8F9) [H00005079-M01] - Analysis of PAX5 over-expressed 293 cell line, cotransfected with PAX5 Validated Chimera RNAi ( Cat # H00005079-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PAX5 monoclonal antibody (M01), clone 8F9 (Cat # H00005079-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Pax5/BSAP Antibody (8F9) [H00005079-M01] - Analysis of PAX5 expression in transfected 293T cell line by PAX5 monoclonal antibody (M01), clone 8F9.Lane 1: PAX5 transfected lysate(42.1 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Pax5/BSAP Antibody (8F9) [H00005079-M01] - PAX5 monoclonal antibody (M01), clone 8F9. Analysis of PAX5 expression in IMR-32.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Pax5/BSAP Antibody (8F9) [H00005079-M01] - Detection limit for recombinant GST tagged PAX5 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Pax5/BSAP Antibody (8F9) [H00005079-M01] - Analysis of monoclonal antibody to PAX5 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 1.5 ug/ml.