LS-C114670

antibody from LSBio

Targeting: CRYAB CRYA2, HSPB5

Western blot (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C114670

Provider

LSBio

Product name

CRYAB / Alpha B Crystallin Antibody (clone OTI3C2) LS-C114670

Antibody type

Monoclonal

Description

Protein A/G purified

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI3C2

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitation(IP) of CRYAB by using monoclonal anti-CRYAB antibodies (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-CRYAB antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation(IP) of CRYAB by using monoclonal anti-CRYAB antibodies (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-CRYAB antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation(IP) of CRYAB by using monoclonal anti-CRYAB antibodies (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-CRYAB antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Carcinoma of bladder tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded lymphoma tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Carcinoma of bladder tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded lymphoma tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Carcinoma of bladder tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded lymphoma tissue using anti-CRYAB mouse monoclonal antibody. (Dilution 1:50).