200-301-E86

antibody from Rockland Immunochemicals, Inc.

Targeting: CRYAB CRYA2, HSPB5

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

200-301-E86

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#200-301-E86, RRID:AB_2611122

Product name

Anti-Alpha B Crystallin (MOUSE) Monoclonal Antibody - 200-301-E86

Antibody type

Monoclonal

Vial size

200 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western Blot of mouse Anti-Alpha B Crystallin Antibody. Lane 1: Rat Brain. Lane 2: Rat Heart. Lane 3: Rat Kidney. Lane 4: Rat liver. Lane 5: Rat lung. Lane 6: Rat Pancreas. Lane 7: Rat skeletal muscle. Lane 8: Rat Spleen. Lane 9: Rat testes. Lane 10:Rat thymus. Lane 11: C2C12 cell line. Lane 12: NIH 3T3 ctrl. Lane 13: PAM212 cell line. Load: 20ug per lane. Primary antibody: AlphaB-Crystallin 1:1000 for overnight at 4°C. Secondary antibody: Goat anti-Mouse IgG HRP antibody at 1:40,000 for 45 min at RT. Block: 5% Blotto overnight at 4°C. Predicted/Observed size: 19.9 kDa, 20 kDa for Alpha B Crystallin.

Validation comment

Western Blot