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- Antibody Data
- Antigen structure
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- Western blot [1]
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- Product number
- NBP1-22410 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-22410, RRID:AB_1659896
- Product name
- Mouse Monoclonal AlphaB Crystallin/CRYAB Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects approx 20kDa (predicted mol.weight is approx 21kDa). Does not cross-react with alpha A-crystallin, Beta L-crystallin, Beta H-crystallin, gamma-crystallin, HSP25, HSP27 or HSP47 proteins.
- Reactivity
- Human, Rat, Bovine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.2 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: AlphaB Crystallin/CRYAB Antibody (3A10.C9) [NBP1-22410] - analysis of Rat Brain, Heart, Kidney, Liver, Pancreas, Skeletal muscle, Spleen, Testes, Thymus cell lysates showing detection of Alpha B Crystallin protein using Mouse Anti-Alpha B Crystallin Monoclonal Antibody, Clone 3A10-C9 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Alpha B Crystallin Monoclonal Antibody at 1:50 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
