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- Antibody Data
- Antigen structure
- References [1]
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- Western blot [2]
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- Product number
- NB120-13497 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB120-13497, RRID:AB_789461
- Product name
- Rabbit Polyclonal AlphaB Crystallin/CRYAB Antibody
- Antibody type
- Polyclonal
- Description
- Affinity purified. Detects approx 22kDa. Does not cross-react with alpha A-crystallin.
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.2 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Synaptic proteins linked to HIV-1 infection and immunoproteasome induction: proteomic analysis of human synaptosomes.
Gelman BB, Nguyen TP
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 2010 Mar;5(1):92-102
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 2010 Mar;5(1):92-102
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Western blot analysis of Human A431, HCT116, HeLa, HepG2, HEK293, HUVEC, Jurkat, MCF7, PC3 and T98G cell lysates showing detection of ~22 kDa Alpha B Crystallin protein using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: A431 cell lysates. Lane 3: HCT116 cell lysates. Lane 4: HeLa cell lysates. Lane 5: HepG2 cell lysates. Lane 6: HEK293 cell lysates. Lane 7: HUVEC cell lysates. Lane 8: Jurkat cell lysates. Lane 9: MCF7 cell lysates. Lane 10: PC3 cell lysates. Lane 11: T98G cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Western blot analysis of Rat Brain cell lysates showing detection of ~22 kDa Alpha B Crystallin protein using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.
