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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- TA500583 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500583, RRID:AB_10585423
- Product name
- CRYAB (Alpha B Crystallin) mouse monoclonal antibody, clone OTI6D11 (formerly 6D11)
- Antibody type
- Monoclonal
- Description
- CRYAB (Alpha B Crystallin) mouse monoclonal antibody, clone OTI6D11 (formerly 6D11)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CRYAB
- Isotype
- IgG
- Antibody clone number
- OTI6D11
- Vial size
- 100 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-CRYAB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (RC202718, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYAB.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-CRYAB monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-CRYAB mouse monoclonal antibody (TA500583) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB(RC202718).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HT29 cells using anti-CRYAB mouse monoclonal antibody (TA500583).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation(IP) of CRYAB by using TrueMab monoclonal anti-CRYAB antibodies (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-CRYAB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CRYAB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500583)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human smooth muscle cancer tissue using anti-CRYAB mouse monoclonal antibody. (Heat-induced epitope retrieval, TA500583). Data courtesy of a collaborative pathologist.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC202718 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CRYAB antibody(TA500583), and then analyzed by flow cytometry.
- Validation comment
- FC
